Recombinant
RabMAb

Anti-PTP epsilon antibody [EPR6715] (ab126788)

Overview

  • Product name
    Anti-PTP epsilon antibody [EPR6715]
    See all PTP epsilon primary antibodies
  • Description
    Rabbit monoclonal [EPR6715] to PTP epsilon
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human PTP epsilon (UniProt P23469).

  • Positive control
    • HL60 treated with TPA, HL60, HeLa and C2C12 cell lysates; Permeabilized HL60 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 71, 75, 81 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
      Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
      Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
    • Tissue specificity
      Expressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain
      The tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity.
    • Post-translational
      modifications
      A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
      Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
      Isoform 1 is glycosylated.
    • Cellular localization
      Cytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp313F1310 antibody
      • FLJ57799 antibody
      • FLJ58245 antibody
      • HPTPE antibody
      • Protein tyrosine phosphatase epsilon antibody
      • Protein tyrosine phosphatase receptor type E antibody
      • Protein tyrosine phosphatase receptor type epsilon antibody
      • Protein tyrosine phosphatase receptor type epsilon polypeptide antibody
      • Protein-tyrosine phosphatase epsilon antibody
      • PTPE antibody
      • Ptpre antibody
      • PTPRE_HUMAN antibody
      • R PTP Epsilon antibody
      • R-PTP-epsilon antibody
      • Receptor-type tyrosine-protein phosphatase epsilon antibody
      see all

    Images

    • All lanes : Anti-PTP epsilon antibody [EPR6715] (ab126788) at 1/1000 dilution

      Lane 1 : HL60 cell lysate, treated with TPA
      Lane 2 : HL60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : C2C12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 71, 75, 81 kDa
    • ab126788 at 1/10 dilution staining PTP epsilon in permeabilized HL60 cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126788 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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