Anti-PTPRK antibody (ab185370)
Key features and details
- Rabbit polyclonal to PTPRK
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTPRK antibody
See all PTPRK primary antibodies -
Description
Rabbit polyclonal to PTPRK -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PTPRK aa 750-900.
Database link: Q15262 -
Positive control
- IHC-P: Human endometrium, prostate, and cerebral cortex tissues. ICC: SiHa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa. -
Tissue specificity
High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains. -
Post-translational
modificationsThis protein undergoes proteolytic processing. -
Cellular localization
Cell junction > adherens junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5796 Human
- Omim: 602545 Human
- SwissProt: Q15262 Human
- Unigene: 155919 Human
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Alternative names
- dJ480J14.2.1 antibody
- DKFZp686C2268 antibody
- DKFZp779N1045 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling PTPRK with ab185370 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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SiHa cells stained for PTPRK (green) using ab185370 at 2 µg/m dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling PTPRK with ab185370 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling PTPRK with ab185370 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling PTPRK with ab185370 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab185370 has been referenced in 1 publication.
- Xu X et al. Downregulation of PTPRK Promotes Cell Proliferation and Metastasis of NSCLC by Enhancing STAT3 Activation. Anal Cell Pathol (Amst) 2019:4265040 (2019). PubMed: 30838170