This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    WB: Inconclusive data due to lysates used. A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230), however no band is observed in human brain or pancreas lysate.

    IF/ICC: see figure below.

    Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
    • Cellular localization
      Type 1 membrane protein
    • Database links
    • Alternative names
      • Pancreatic carcinoma phosphatase 2 antibody
      • PCP-2 antibody
      • Protein-tyrosine phosphatase J antibody
      • PTP-J antibody
      • PTPRU antibody
      • R-PTP-U antibody
      • Receptor-type tyrosine-protein phosphatase U antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Human kidney lysate
      Lane 3 : Human pancreas lysate
      Lane 4 : Human heart lysate
      Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
      Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
      Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
      Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Performed under reducing conditions.

      Exposure time : 5 minutes

      A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).

    • Image courtesy of www.proteinatlas.org
      ab13223 staining PTP lambda in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
    • ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    References

    This product has been referenced in:
    • Zhu Z  et al. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility. Carcinogenesis 35:1901-10 (2014). Read more (PubMed: 24876153) »
    • Hyun SW  et al. Diverse injurious stimuli reduce protein tyrosine phosphatase-µ expression and enhance epidermal growth factor receptor signaling in human airway epithelia. Exp Lung Res 37:327-43 (2011). Read more (PubMed: 21649524) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab13223.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up