This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product name
    Anti-PTP lambda antibody
  • Description
    Rabbit polyclonal to PTP lambda

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
    Rabbit
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from residues 850 - 950 of Human PTP lambda.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    WB: Inconclusive data due to lysates used. A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230), however no band is observed in human brain or pancreas lysate.

    IF/ICC: see figure below.

    Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
    • Cellular localization
      Type 1 membrane protein
    • Database links
    • Alternative names
      • Pancreatic carcinoma phosphatase 2 antibody
      • PCP-2 antibody
      • Protein-tyrosine phosphatase J antibody
      • PTP-J antibody
      • PTPRU antibody
      • R-PTP-U antibody
      • Receptor-type tyrosine-protein phosphatase U antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Wester Blot with ab13223 Rabbit polyclonal to PTP lambda (1/500)
      Secondary: Goat anti-rabbit IgG ab6721 (1/5000)
      Exposure time: 5 mins
      Expected molecular weight: 160 kDa

      Lanes 1 to 8: 20µg of cell lysate per lane
      Lanes 5 to 8: blocking peptide used at 1µg/ml

      Lane 1: Human Brain Lysate
      Lane 2: Human Kidney Lysate
      Lane 3: Human Pancreas Lysate
      Lane 4: Human Heart Lysate
      Lane 5: Human Brain Lysate + blocking peptide (ab13230)
      Lane 6: Human Kidney Lysate + blocking peptide (ab13230)
      Lane 7: Human Pancreas Lysate + blocking peptide (ab13230)
      Lane 8: Human Heart Lysate + blocking peptide (ab13230)

      A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).

    • Image courtesy of www.proteinatlas.org
      ab13223 staining PTP lambda in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
    • ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    References

    This product has been referenced in:
    • Zhu Z  et al. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility. Carcinogenesis 35:1901-10 (2014). Read more (PubMed: 24876153) »
    • Hyun SW  et al. Diverse injurious stimuli reduce protein tyrosine phosphatase-µ expression and enhance epidermal growth factor receptor signaling in human airway epithelia. Exp Lung Res 37:327-43 (2011). Read more (PubMed: 21649524) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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