WB: Inconclusive data due to lysates used. A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230), however no band is observed in human brain or pancreas lysate.
IF/ICC: see figure below.
Tested in peptide ELISA.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
Receptor-type tyrosine-protein phosphatase U antibody
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Western blot - Anti-PTP lambda antibody (ab13223)
Wester Blot with ab13223 Rabbit polyclonal to PTP lambda (1/500) Secondary: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 5 mins Expected molecular weight: 160 kDa
Lanes 1 to 8: 20µg of cell lysate per lane Lanes 5 to 8: blocking peptide used at 1µg/ml
Lane 1: Human Brain Lysate Lane 2: Human Kidney Lysate Lane 3: Human Pancreas Lysate Lane 4: Human Heart Lysate Lane 5: Human Brain Lysate + blocking peptide (ab13230) Lane 6: Human Kidney Lysate + blocking peptide (ab13230) Lane 7: Human Pancreas Lysate + blocking peptide (ab13230) Lane 8: Human Heart Lysate + blocking peptide (ab13230)
A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
Image courtesy of www.proteinatlas.org ab13223 staining PTP lambda in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
This product has been referenced in:
Zhu Z et al. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility. Carcinogenesis35:1901-10 (2014).
Read more (PubMed: 24876153) »
Hyun SW et al. Diverse injurious stimuli reduce protein tyrosine phosphatase-µ expression and enhance epidermal growth factor receptor signaling in human airway epithelia. Exp Lung Res37:327-43 (2011).
Read more (PubMed: 21649524) »