Anti-PTP zeta/ Phosphacan antibody (ab126497)

Overview

  • Product name
    Anti-PTP zeta/ Phosphacan antibody
    See all PTP zeta/ Phosphacan primary antibodies
  • Description
    Rabbit polyclonal to PTP zeta/ Phosphacan
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LFRHLHTVSQ ILPQVTSATE SDKVPLHASL PVAGGDLLLE PSLAQYSDVL STTHAASETL EFGSESGVLY KTLMFSQVEP PSSDAMMHAR SSGPEPSYAL SDNEGSQHIF TVSYSSAIPV HDSVGVTYQG SLFSGPSHIP IPKSSLIT corresponding to internal amino acids 831-978 of Human PTP zeta/ Phosphacan.

  • Positive control
    • Human cerebral cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 255 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in the regulation of specific developmental processes in the CNS.
  • Tissue specificity
    Specifically expressed in the central nervous system, where it is localized in the Purkinje cell layer of the cerebellum, the dentate gyrus, and the subependymal layer of the anterior horn of the lateral ventricle. Developmentally regulated in the brain.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.
    Contains 1 alpha-carbonic anhydrase domain.
    Contains 1 fibronectin type-III domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3F8 chondroitin sulfate proteoglycan antibody
    • 3H1 keratan sulfate proteoglycan antibody
    • HPTPZ antibody
    • HPTPzeta antibody
    • Phosphacan antibody
    • Protein tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • Protein tyrosine phosphatase, receptor type, Z polypeptide 1 antibody
    • Protein tyrosine phosphatase, receptor type, zeta polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • PTP-ZETA antibody
    • PTP18 antibody
    • PTPRZ antibody
    • PTPRZ_HUMAN antibody
    • Ptprz1 antibody
    • PTPZ antibody
    • R PTP zeta 2 antibody
    • R-PTP-zeta antibody
    • R-PTP-zeta-2 antibody
    • Receptor type tyrosine phosphatase beta/zeta antibody
    • Receptor-type tyrosine-protein phosphatase zeta antibody
    • RPTP-BETA antibody
    • RPTPB antibody
    • RPTPbeta antibody
    see all

Images

  • Anti-PTP zeta/ Phosphacan antibody (ab126497) + Human brain tissue

    Predicted band size : 255 kDa
  • Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab126497 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126497 at 1/50 dilution staining PTP zeta/ Phosphacan in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Baek JH  et al. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease. J Clin Invest 125:3198-214 (2015). Read more (PubMed: 26121749) »
  • Pantazopoulos H  et al. Proteoglycan abnormalities in olfactory epithelium tissue from subjects diagnosed with schizophrenia. Schizophr Res 150:366-72 (2013). IHC ; Human . Read more (PubMed: 24035561) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain - Amygdala and ECx)
Specification
Brain - Amygdala and ECx
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X(0.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 01 2012

I am very pleased to hear you would like to accept our offer and test ab126497 inIHC-FrFl. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for [IHC-FrFl and include this code in...

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