Overview

  • Product name
    Anti-PTP1B antibody [3A7]
    See all PTP1B primary antibodies
  • Description
    Mouse monoclonal [3A7] to PTP1B
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant human protein purified from E.coli

  • Positive control
    • HepG2 Cell lysates. 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab21257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 50 kDa.

Target

  • Function
    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

Images

  • All lanes : Anti-PTP1B antibody [3A7] (ab21257) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Hep3B cell lysate

    Predicted band size: 50 kDa



    Ladder M.W(kDa):7,17,25,30,46,58,80,175

  • All lanes : Anti-PTP1B antibody [3A7] (ab21257) at 1/2000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : 293T cell lysates

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

References

ab21257 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Liver lysate)
Specification
Liver lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type
Direct
Username

Abcam user community

Verified customer

Submitted Dec 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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