Anti-PTP1B antibody (ab189179)
Key features and details
- Rabbit polyclonal to PTP1B
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PTP1B antibody
See all PTP1B primary antibodies -
Description
Rabbit polyclonal to PTP1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide within Human PTP1B aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002818.1).
Database link: P18031 -
Positive control
- Human kidney tissue; Extracts from COS7 cells, treated with UV 30'.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5770 Human
- Omim: 176885 Human
- SwissProt: P18031 Human
- Unigene: 417549 Human
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Alternative names
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PTP1B with ab189179 at 10 µg/ml.
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All lanes : Anti-PTP1B antibody (ab189179) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated with UV 30'
Lane 2 : Extracts from COS7 cells, treated with UV 30' with immunizing peptide
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab189179 has been referenced in 6 publications.
- Shi H et al. Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice. J Neuroinflammation 17:77 (2020). PubMed: 32127019
- Shi H et al. ß-glucan attenuates cognitive impairment via the gut-brain axis in diet-induced obese mice. Microbiome 8:143 (2020). PubMed: 33008466
- Shu XS et al. Loss of ß-catenin via activated GSK3ß causes diabetic retinal neurodegeneration by instigating a vicious cycle of oxidative stress-driven mitochondrial impairment. Aging (Albany NY) 12:13437-13462 (2020). PubMed: 32575075
- Guerville M et al. Chronic refined low-fat diet consumption reduces cholecystokinin satiation in rats. Eur J Nutr 58:2497-2510 (2019). PubMed: 30069617
- Garner KL et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep 8:3902 (2018). PubMed: 29500363
- Borges Bde C et al. Protein tyrosine phosphatase-1B contributes to LPS-induced leptin resistance in male rats. Am J Physiol Endocrinol Metab 308:E40-50 (2015). PubMed: 25352433