Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239)

Overview

  • Product nameAnti-PTP1B (phospho S378) antibody [EP1840Y]
    See all PTP1B primary antibodies
  • Description
    Rabbit monoclonal [EP1840Y] to PTP1B (phospho S378)
  • Specificityab76239 detects PTP1B phosphorylated on serine 378.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding serine 378 of human PTP1B

  • Positive control
    • HeLa cytoplasmic or nuclear lysate treated with calyculin A Human breast carcinoma tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notesICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
    WB: 1/500000 - 1/1000000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Post-translational
      modifications
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho S378) antibody [EP1840Y] images

    • All lanes : Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239) at 1/1000000 dilution

      Lane 1 : HeLa cell lysates - untreated
      Lane 2 : HeLa cell lysates - treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab76239, at a 1/50 dilution, staining human PTP1B (phospho S378) in breast carcinoma by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    References for Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239)

    ab76239 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76239.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"