Anti-PTP1B (phospho S50) antibody (ab88472)

Overview

  • Product name
    Anti-PTP1B (phospho S50) antibody
    See all PTP1B primary antibodies
  • Description
    Rabbit polyclonal to PTP1B (phospho S50)
  • Specificity
    ab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to amino acids surrounding Ser 50 of Human PTP1B

  • Positive control
    • Lysate from Human A431 and Jurkat cells treated with Calyculin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab88472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Post-translational
      modifications
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Images

    • All lanes : Anti-PTP1B (phospho S50) antibody (ab88472) at 1/1000 dilution

      Lane 1 : lysate from untreated Jurkat cells
      Lane 2 : lysate from Jurkat cells treated with calyculin A (100 nM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References

    ab88472 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (muscle cells)
    Loading amount
    40 µg
    Specification
    muscle cells
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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