Anti-PTP1B (phospho S50) antibody (ab88472)

Overview

  • Product nameAnti-PTP1B (phospho S50) antibody
    See all PTP1B primary antibodies
  • Description
    Rabbit polyclonal to PTP1B (phospho S50)
  • Specificityab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to amino acids surrounding Ser 50 of Human PTP1B

  • Positive control
    • Lysate from Human A431 and Jurkat cells treated with Calyculin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab88472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Post-translational
      modifications
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho S50) antibody images

    • All lanes : Anti-PTP1B (phospho S50) antibody (ab88472) at 1/1000 dilution

      Lane 1 : lysate from untreated Jurkat cells
      Lane 2 : lysate from Jurkat cells treated with calyculin A (100 nM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References for Anti-PTP1B (phospho S50) antibody (ab88472)

    ab88472 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (muscle cells)
    Loading amount 40 µg
    Specification muscle cells
    Gel Running Conditions Reduced Denaturing (7.5)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Verified customer

    Submitted Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"