The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.
Post-translational modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.