Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320)

Overview

  • Product name
    Anti-PTP1B (phospho S50) antibody [EP1838Y]
    See all PTP1B primary antibodies
  • Description
    Rabbit monoclonal [EP1838Y] to PTP1B (phospho S50)
  • Specificity
    This antibody detects PTP1B when phosphorylated at Serine 50.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B.

  • Positive control
    • Hela cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

Images

  • All lanes : Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320) at 1/5000 dilution

    Lane 1 : Hela cell lysate - untreated cells
    Lane 2 : Hela cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References

ab62320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab62320.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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