Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320)

Overview

  • Product nameAnti-PTP1B (phospho S50) antibody [EP1838Y]
    See all PTP1B primary antibodies
  • Description
    Rabbit monoclonal [EP1838Y] to PTP1B (phospho S50)
  • SpecificityThis antibody detects PTP1B when phosphorylated at Serine 50.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B.

  • Positive control
    • Hela cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

Anti-PTP1B (phospho S50) antibody [EP1838Y] images

  • All lanes : Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320) at 1/5000 dilution

    Lane 1 : Hela cell lysate - untreated cells
    Lane 2 : Hela cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320)

ab62320 has not yet been referenced specifically in any publications.

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