Anti-PTP1B (phospho Y152) antibody (ab88473)

Overview

  • Product nameAnti-PTP1B (phospho Y152) antibody
    See all PTP1B primary antibodies
  • Description
    Rabbit polyclonal to PTP1B (phospho Y152)
  • SpecificityDoes not react with control cells or after alkaline phosphatase treatment of blots.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acids surrounding Tyr 152 of Human PTP1B.

  • Positive control
    • Pervanadate-treated Human A431 and Jurkat cells or Mouse SYF cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Post-translational
      modifications
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho Y152) antibody images

    • All lanes : Anti-PTP1B (phospho Y152) antibody (ab88473) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature)

      Lane 1 : Mouse SYF cSrc-transformed cells, untreated
      Lane 2 : Mouse SYF cSrc-transformed cells treated with pervanadate (1 mM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References for Anti-PTP1B (phospho Y152) antibody (ab88473)

    ab88473 has not yet been referenced specifically in any publications.

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