Anti-PTP1B (phospho Y152) antibody (ab88473)

Overview

  • Product name
    Anti-PTP1B (phospho Y152) antibody
    See all PTP1B primary antibodies
  • Description
    Rabbit polyclonal to PTP1B (phospho Y152)
  • Specificity
    Does not react with control cells or after alkaline phosphatase treatment of blots.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acids surrounding Tyr 152 of Human PTP1B.

  • Positive control
    • Pervanadate-treated Human A431 and Jurkat cells or Mouse SYF cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Cross adsorbed to a phospho-tyrosine peptide, then affinity purified with phospho PTP1B (Tyr 152) peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Post-translational
      modifications
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho Y152) antibody images

    • All lanes : Anti-PTP1B (phospho Y152) antibody (ab88473) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature)

      Lane 1 : Mouse SYF cSrc-transformed cells, untreated
      Lane 2 : Mouse SYF cSrc-transformed cells treated with pervanadate (1 mM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References for Anti-PTP1B (phospho Y152) antibody (ab88473)

    ab88473 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab88473.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up