Anti-PTP4A1 antibody (ab1368)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/2000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa in HeLa and A549 lysates (predicted molecular weight: 20 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.
    • Tissue specificityExpressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family.
      Contains 1 tyrosine-protein phosphatase domain.
    • Developmental stageExpressed in fetal liver.
    • Post-translational
      modifications
      Farnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus.
    • Cellular localizationCell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • HH 72 antibody
      • HH72 antibody
      • Hypothetical protein DKFZp779M0721 antibody
      • Phosphatase of regenerating liver 1 antibody
      • PRL 1 antibody
      • PRL-1 antibody
      • PRL1 antibody
      • Protein tyrosine phosphatase PTPCAAX1 antibody
      • Protein tyrosine phosphatase type IVA 1 antibody
      • Protein tyrosine phosphatase type IVA member 1 antibody
      • Protein-tyrosine phosphatase 4a1 antibody
      • Protein-tyrosine phosphatase of regenerating liver 1 antibody
      • Protein-tyrosine phosphatase, type 4A, 1 antibody
      • PTP(CAAXI) antibody
      • Ptp4a1 antibody
      • PTPCAAX1 antibody
      • TP4A1_HUMAN antibody
      see all

    Anti-PTP4A1 antibody images

    • Anti-PTP4A1 antibody (ab1368) at 2 µg/ml + Human A549 lysate (35µg in RIPA buffer)

      Predicted band size : 20 kDa

    References for Anti-PTP4A1 antibody (ab1368)

    ab1368 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (COS7 extract expressing HA-tagged PTP4A1.)
    Loading amount 10 µg
    Specification COS7 extract expressing HA-tagged PTP4A1.
    Gel Running Conditions Reduced Denaturing
    Blocking step Blocking One as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2009

    Thank you for your enquiry. We have withdrawn ab1368from our product range. We do hope that this does not cause you too much inconvenience. Have you purchased this antibody? Have you done any experiments with it?

    Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"