• Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab12550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Relevance
    PTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis.
  • Cellular localization
    Cytoplasmic, Cytoskeleton
  • Database links
  • Alternative names
    • Protein tyrosine phosphatase D1 antibody
    • Protein tyrosine phosphatase non receptor type 21 antibody
    • PTPN 21 antibody
    • PTPN21 antibody
    • PTPNL antibody
    • PTPRL10 antibody
    • Tyrosine protein phosphatase non receptor type 21 antibody
    see all

Anti-PTPD1 antibody images

  • All lanes : Anti-PTPD1 antibody (ab12550) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 133 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Image courtesy of Human Protein Atlas

    ab12550 staining PTPD1 in Human kidney. Paraffin embedded human skin tissue was incubated with ab12550 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab12550 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

References for Anti-PTPD1 antibody (ab12550)

ab12550 has not yet been referenced specifically in any publications.

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