Overview

  • Product name
    Anti-PTPLAD2 antibody
  • Description
    Rabbit polyclonal to PTPLAD2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (LLHIYVGIES NHLLPRFLQL TERIIILFVV ITSQEEVQEK YVVCVLFVFW) of human PTPLAD2 (NP_001010915).

  • Positive control
    • 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-PTPLAD2 antibody (ab82949) at 1 µg/ml (in 5% skim milk / PBS) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 66 kDa. We are unsure as to the identity of these extra bands.

References

ab82949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tissue microarray with several normal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA buffer, pH 9.0
Permeabilization
No
Specification
Tissue microarray with several normal tissues
Blocking step
Blocking agent from Novolink Max Polymer Detection System as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde
Username

Lucas Bidinotto

Verified customer

Submitted Mar 11 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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