Overview

  • Product nameAnti-PTPN12 antibody
    See all PTPN12 primary antibodies
  • Description
    Rabbit polyclonal to PTPN12
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 375 and 425 of human protein PTPN12 (NP_002826.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab76936 was affinity purified using an epitope specific to PTPN12 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 ug/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 88 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein tyrosine phosphatase G1 antibody
      • Protein tyrosine phosphatase non receptor type 12 antibody
      • Protein-tyrosine phosphatase G1 antibody
      • PTN12_HUMAN antibody
      • PTP PEST antibody
      • PTP-PEST antibody
      • PTPG1 antibody
      • Ptpn12 antibody
      • Tyrosine protein phosphatase non receptor type 12 antibody
      • Tyrosine-protein phosphatase non-receptor type 12 antibody
      see all

    Anti-PTPN12 antibody images

    • All lanes : Anti-PTPN12 antibody (ab76936) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 88 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab76936 at 1 ug/ml (used at 3µg/mg lysate for IP).
      Lane 2: control IgG.
      Detection: Chemiluminescence with an exposure time of 1 second.

    References for Anti-PTPN12 antibody (ab76936)

    ab76936 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76936.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"