Overview

  • Product name
  • Description
    Rabbit polyclonal to PTPN12
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and 550 of human PTPN12 (NP_002826.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab76937 was affinity purified using an epitope specific to PTPN12 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein tyrosine phosphatase G1 antibody
    • Protein tyrosine phosphatase non receptor type 12 antibody
    • Protein-tyrosine phosphatase G1 antibody
    • PTN12_HUMAN antibody
    • PTP PEST antibody
    • PTP-PEST antibody
    • PTPG1 antibody
    • Ptpn12 antibody
    • Tyrosine protein phosphatase non receptor type 12 antibody
    • Tyrosine-protein phosphatase non-receptor type 12 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling PTPN12 with ab76937 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PTPN12 antibody (ab76937) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Lane 1: ab76937, at 1 µg/mg, staining PTPN12 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitate was performed by using ab76937 at 3µg/mg of lysate.
    Lane 2: Control IgG.
    Detection: Chemiluminescence with an exposure time of 1 second.

References

ab76937 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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