Purification notesab76939 was affinity purified using an epitope specific to PTPN12 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals
1.0 mg of IgG.
Lane 1: ab76939, at 1 µg/mg, staining PTPN12 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab76939 at 3µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-PTPN12 antibody (ab76939)
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