Anti-PTPN12 antibody - N-terminal (ab154892)

Overview

  • Product name
    Anti-PTPN12 antibody - N-terminal
    See all PTPN12 primary antibodies
  • Description
    Rabbit polyclonal to PTPN12 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within N terminal amino acids 1-207 of PTPN12 (Q05209)

  • Positive control
    • A549, HeLa or HepG2 lysate, HeLa cells, D54 xenograft, RT2 xenograft or Human ulcerative colitis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-PTPN12 antibody - N-terminal (ab154892) at 1/5000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human D54 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
  • Immunohistochemical analysis of paraffin embedded Human RT2 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
  • Immunohistochemical analysis of paraffin embedded Human ulcerative colitis tissue labeling PTPN12 with ab154892 at a 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PTPN12 with ab154892 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)

References

ab154892 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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