The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/40 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate with primary antibody for 60 minutes at 25°C.
RelevancePTPN13 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as I-kappa-B-alpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway.
Immunohistochemical staining (Paraffin section) for PTPN13 (Fas-associated phosphatase 1) using ab49446 with normal Human thyroid as positive control. Note intense cytoplasmic staining of thyroid epithelial cells.
References for Anti-PTPN13 antibody [AC21] (ab49446)
This product has been referenced in:
Glondu-Lassis M et al. PTPL1/PTPN13 regulates breast cancer cell aggressiveness through direct inactivation of Src kinase. Cancer Res70:5116-26 (2010).
Read more (PubMed: 20501847) »