Overview

  • Product name
  • Description
    Mouse monoclonal to PTPRS
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: EPPRFIKEPK DQIGVSGGVA SFVCQATGDP KPRVTWNKKG KKVNSQRFET IEFDESAGAV LRIQPLRTPR DENVYECVAQ NSVGEITVHA KLTVLRGP, corresponding to amino acids 1-129 of Human PTPRS

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab55640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 217 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Interacts with LAR-interacting protein LIP.1.
  • Tissue specificity
    Detected in all tissues tested except for placenta and liver.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.
    Contains 8 fibronectin type-III domains.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Post-translational
    modifications
    A cleavage occurs, separating the extracellular domain from the transmembrane segment. This process called 'ectodomain shedding' is thought to be involved in receptor desensitization, signal transduction and/or membrane localization.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein tyrosine phosphatase PTPsigma antibody
    • Protein tyrosine phosphatase receptor type S antibody
    • Protein tyrosine phosphatase receptor type sigma antibody
    • Ptprs antibody
    • PTPRS_HUMAN antibody
    • PTPSIGMA antibody
    • R PTP sigma antibody
    • R-PTP-S antibody
    • R-PTP-sigma antibody
    • Receptor-type tyrosine-protein phosphatase S antibody
    • Receptor-type tyrosine-protein phosphatase sigma antibody
    see all

Images



  • Predicted band size : 217 kDa
  • Anti-PTPRS antibody (ab55640) + immunogen

    Predicted band size : 217 kDa
  • Overlay histogram showing HeLa cells stained with ab55640 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55640, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

ab55640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Gel Running Conditions
Reduced Denaturing (Nupage precast gel bis-tris 4-12% NP0322BOX)
Loading amount
60 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Vera Medvedeva

Verified customer

Submitted Apr 10 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4-12% gel, MES buffer, 4xLaemmli Sample buffer)
Sample
Mouse Cell lysate - whole cell (Brain lysate)
Specification
Brain lysate
Treatment
4xLaemmli Sample buffer, 80C boil 10 minutes
Blocking step
SuperBlocking as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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