Overview

  • Product nameAnti-PTRF antibody
    See all PTRF primary antibodies
  • Description
    Rabbit polyclonal to PTRF
  • Tested applicationsSuitable for: IP, IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, African Green Monkey, Chinese Hamster
    Predicted to work with: Dog
  • Immunogen

    A region within synthetic peptide: PEKEGDELGE GERPEDDTAA IELSSDEAVE VEEVIEESRA ERIKRSGLRR, corresponding to internal sequence amino acids 181-230 of Mouse PTRF

  • Positive control
    • NIH3T3 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200 - 1/250. (for 20 hrs - total 250 ug protein)
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
  • Involvement in diseaseCongenital generalized lipodystrophy 4
  • Sequence similaritiesBelongs to the PTRF/SDPR family.
  • Post-translational
    modifications
    Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
    Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
  • Cellular localizationMembrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310075E07Rik antibody
    • AW546441 antibody
    • Cavin 1 antibody
    • Cavin antibody
    • Cavin-1 antibody
    • CAVIN1 antibody
    • CGL4 antibody
    • FKSG13 antibody
    • FLJ90031 antibody
    • MGC118550 antibody
    • OTTMUSP00000002049 antibody
    • Polymerase I and transcript release factor antibody
    • Ptrf antibody
    • PTRF_HUMAN antibody
    • RNA polymerase I and transcript release factor antibody
    • RP23-279L23.6 antibody
    • TTF I interacting peptide 12 antibody
    see all

Anti-PTRF antibody images

  • Anti-PTRF antibody (ab48824) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 1 hour at 22°C.

    See Abreview

  • Anti-PTRF antibody (ab48824) at 1/2000 dilution + Rat C6 cells, whole cell lysate at 25 µg

    Secondary
    HRP conjugated goat anti-rabbit antibody at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Lane 1 : Anti-PTRF antibody (ab48824) at 5 µg/ml
    Lane 2 : Anti-PTRF antibody (ab48824) at 2.5 µg/ml
    Lane 3 : Anti-PTRF antibody (ab48824) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from mouse NIH3T3 cells
    Lane 2 : Cell lysate prepared from mouse NIH3T3 cells
    Lane 3 : Cell lysate prepared from mouse NIH3T3 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 43 kDa
  • ab48824 (4µg/ml) staining PTRF in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-PTRF antibody (ab48824)

This product has been referenced in:
  • Shvets E  et al. Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids. Nat Commun 6:6867 (2015). Human . Read more (PubMed: 25897946) »
  • Tome ME  et al. Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. J Neurochem 134:200-10 (2015). Read more (PubMed: 25832806) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Pericytes)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Pericytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 19 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK)
Permeabilization Yes - 0.1% Triton in PBS
Specification MDCK
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Jan 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (pericytes)
Permeabilization Yes - 0.1% Triton in PBS
Specification pericytes
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Dec 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (cos-7)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification cos-7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 07 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (Brain endothelial cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Brain endothelial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 29 2015

Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 10 µg
Specification Hela cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 29 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (bEnd.3)
Permeabilization Yes - 0.1% Triton in PBS
Specification bEnd.3
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Jul 28 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Permeabilization Yes - 0.1% Triton in PBS
Specification Hela
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Jul 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (L6 cell line)
Total protein in input 200 µg
Specification L6 cell line
Immuno-precipitation step Protein A/G
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Submitted Jun 04 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Chinese Hamster Cell lysate - whole cell (CHO cell line)
Total protein in input 200 µg
Specification CHO cell line
Immuno-precipitation step Protein A/G
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Submitted Jun 04 2009

1-10 of 24 Abreviews or Q&A

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