Overview

  • Product nameAnti-PTRF antibody
    See all PTRF primary antibodies
  • Description
    Rabbit polyclonal to PTRF
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Opossum, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 125 and 175 of human PTRF (NP_036364.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionPlays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
  • Involvement in diseaseCongenital generalized lipodystrophy 4
  • Sequence similaritiesBelongs to the PTRF/SDPR family.
  • Post-translational
    modifications
    Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
    Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
  • Cellular localizationMembrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310075E07Rik antibody
    • AW546441 antibody
    • Cavin 1 antibody
    • Cavin antibody
    • Cavin-1 antibody
    • CAVIN1 antibody
    • CGL4 antibody
    • FKSG13 antibody
    • FLJ90031 antibody
    • MGC118550 antibody
    • OTTMUSP00000002049 antibody
    • Polymerase I and transcript release factor antibody
    • Ptrf antibody
    • PTRF_HUMAN antibody
    • RNA polymerase I and transcript release factor antibody
    • RP23-279L23.6 antibody
    • TTF I interacting peptide 12 antibody
    see all

Anti-PTRF antibody images

  • All lanes : Anti-PTRF antibody (ab76919) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 43 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Western Blot of Immunprecipitate.
    ab76919 at 1µg/ml (for WB) staining PTRF in HeLa whole cell lysate,
    immunoprecipitated using ab76919 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References for Anti-PTRF antibody (ab76919)

ab76919 has not yet been referenced specifically in any publications.

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