FunctionPlays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Involvement in diseaseCongenital generalized lipodystrophy 4
Sequence similaritiesBelongs to the PTRF/SDPR family.
Post-translational modificationsPhosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
Cellular localizationMembrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
Detection of PTRF by Western Blot of Immunprecipitate.
ab76920 at 1µg/ml staining PTRF in HeLa whole cell lysate immunoprecipitated using ab76920 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
References for Anti-PTRF antibody (ab76920)
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