Overview

  • Product nameAnti-PTRH2 antibody
    See all PTRH2 primary antibodies
  • Description
    Rabbit polyclonal to PTRH2
  • SpecificityThis antibody is specific for PTRH2.
  • Tested applicationsSuitable for: WB, IHC (PFA fixed), ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of human PTRH2.

  • Positive control
    • A20 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab36990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC (PFA fixed)
ICC/IF
  • Application notesICC/IF: Use at a concentration of 2 µg/ml.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). Can be blocked with PTRH2 peptide (ab39732).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
      Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
    • Sequence similaritiesBelongs to the PTH2 family.
    • Cellular localizationMitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bcl 2 inhibitor of transcription 1 antibody
      • Bcl-2 inhibitor of transcription 1 antibody
      • BIT 1 antibody
      • BIT1 antibody
      • CGI 147 antibody
      • CGI147 antibody
      • mitochondrial antibody
      • Peptidyl tRNA hydrolase 2 mitochondrial antibody
      • Peptidyl-tRNA hydrolase 2 antibody
      • PTH 2 antibody
      • PTH2 antibody
      • PTH2_HUMAN antibody
      • PTRH 2 antibody
      • PTRH2 antibody
      see all

    Anti-PTRH2 antibody images

    • Lane 1 : Anti-PTRH2 antibody (ab36990) at 1 µg/ml
      Lane 2 : Anti-PTRH2 antibody (ab36990) at 2 µg/ml
      Lane 3 : Anti-PTRH2 antibody (ab36990) at 4 µg/ml

      Lane 1 : A20 cell lysate.
      Lane 2 : A20 cell lysate.
      Lane 3 : A20 cell lysate.

      Lysates/proteins at 15 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/10000 dilution

      Predicted band size : 20 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      The arrow next to the image points to the PTRH2 band.
    • ab36990 at 2µg/ml staining PTRH2 in 3T3 cells by ICC/IF

    • Immunofluorescence of Bit1 in 3T3 cells using ab36990 at 20 ug/ml.

    References for Anti-PTRH2 antibody (ab36990)

    This product has been referenced in:
    • Duthie KA  et al. Proteomics analysis of interleukin (IL)-7-induced signaling effectors shows selective changes in IL-7Ralpha449F knock-in T cell progenitors. Mol Cell Proteomics 6:1700-10 (2007). WB ; Mouse . Read more (PubMed: 17602185) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab36990.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"