The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
ICC/IF: Use at a concentration of 2 µg/ml.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). Can be blocked with PTRH2 peptide (ab39732).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.