Overview

  • Product nameAnti-PTRH2 antibody
    See all PTRH2 primary antibodies
  • Description
    Rabbit polyclonal to PTRH2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the centre of human PTRH2.

  • Positive control
    • Daudi cell lysate. Human small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with PTRH2 peptide (ab176553).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionThe natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
    Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
  • Sequence similaritiesBelongs to the PTH2 family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl 2 inhibitor of transcription 1 antibody
    • Bcl-2 inhibitor of transcription 1 antibody
    • BIT 1 antibody
    • BIT1 antibody
    • CGI 147 antibody
    • CGI147 antibody
    • mitochondrial antibody
    • Peptidyl tRNA hydrolase 2 mitochondrial antibody
    • Peptidyl-tRNA hydrolase 2 antibody
    • PTH 2 antibody
    • PTH2 antibody
    • PTH2_HUMAN antibody
    • PTRH 2 antibody
    • PTRH2 antibody
    see all

Anti-PTRH2 antibody images

  • Lane 1 : Anti-PTRH2 antibody (ab62554) at 1 µg/ml
    Lane 2 : Anti-PTRH2 antibody (ab62554) at 2 µg/ml
    Lane 3 : Anti-PTRH2 antibody (ab62554) at 4 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ab62554, at 10µg/ml, staining PTRH2 in human small intestine by immunohistochemistry using paraffin-embedded tissue.
  • Immunofluorescence of Bit1 in Human Small Intestine cells using ab62554 at 20 ug/ml.

References for Anti-PTRH2 antibody (ab62554)

ab62554 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.
I can confirm that the exact immunogen sequence is proprietary, but it is from within aa51-100 of NP_057161.
I hope this information is helpful and wish you good luck with your research project.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"