The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 19,21 kDa (predicted molecular weight: 19 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt,IHC-P or IP.
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.