Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462)

Overview

  • Product nameAnti-PTS (phospho S19) antibody [EPR1517(2)]
    See all PTS primary antibodies
  • Description
    Rabbit monoclonal [EPR1517(2)] to PTS (phospho S19)
  • Specificityab109462 only detects PTS phosphorylated at Serine 19.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 19 of Human PTS.

  • Positive control
    • HUVEC cell lysates; Human brain tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionInvolved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
    • PathwayCofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
    • Involvement in diseaseDefects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.
    • Sequence similaritiesBelongs to the PTPS family.
    • Post-translational
      modifications
      Phosphorylation of Ser-19 is required for maximal enzyme activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6 pyruvoyl tetrahydrobiopterin synthase antibody
      • 6 pyruvoyl tetrahydropterin synthase antibody
      • 6 pyruvoyltetrahydropterin synthase antibody
      • 6-pyruvoyl tetrahydrobiopterin synthase antibody
      • EC 4.2.3.12 antibody
      • FLJ97081 antibody
      • OTTHUMP00000235385 antibody
      • PTP synthase antibody
      • PTPS antibody
      • PTPS_HUMAN antibody
      • PTS antibody
      see all

    Anti-PTS (phospho S19) antibody [EPR1517(2)] images

    • All lanes : Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution

      Lane 1 : HUVEC cell lysates
      Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 16 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109462, at a 1/250 dilution, staining PTS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109462, at a 1/250 dilution, staining PTS (phospho S19) in HeLa cells by Immunofluorescence.

    References for Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462)

    ab109462 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"