The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionBinds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.
ab111002, at 3.75µg/ml, staining PU.1/Spi1 in formalin-fixed, paraffin-embedded Human Colon lymphoid aggregate by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab111002, at 3.75µg/ml, staining PU.1/Spi1 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-PU.1/Spi1 antibody (ab111002)
has not yet been referenced specifically in any publications.
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