The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionBinds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.
Anti-PU.1/Spi1 antibody (ab88082) at 5 µg/ml + Raw 264.7 cell lysate at 25 µg
Secondary Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 31 kDa Observed band size : 31 kDa
Flow Cytometry-Anti-PU.1/Spi1 antibody(ab88082)
Overlay histogram showing K562 cells stained with ab88082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88082, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-PU.1/Spi1 antibody (ab88082)
This product has been referenced in:
Sellgren CM et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry22:170-177 (2017).
Read more (PubMed: 27956744) »