Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)


  • Product nameAnti-PU.1/Spi1 antibody [EPR3158Y]
    See all PU.1/Spi1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3158Y] to PU.1/Spi1
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminal of human PU.1/Spi1

  • Positive control
    • Daudi and U937 cell lysates. Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab76543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000 - 1/20000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionBinds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • Sequence similaritiesBelongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Anti-PU.1/Spi1 antibody [EPR3158Y] images

    • ab76543 showing positive staining in Normal spleen tissue.

    • ab76543, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.
    • All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilution

      Lane 1 : Daudi cell lysates
      Lane 2 : U937 cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    • ab76543 showing positive staining in Normal lung lymphocytes staining tissue.

    • ab76543 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

    This product has been referenced in:
    • Mohr S  et al. Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia. Cancer Cell 31:549-562.e11 (2017). IHC . Read more (PubMed: 28399410) »
    • Frank CL  et al. HDAC inhibitors cause site-specific chromatin remodeling at PU.1-bound enhancers in K562 cells. Epigenetics Chromatin 9:15 (2016). WB ; Human . Read more (PubMed: 27087856) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse prefrontal cortex)
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% BisTris)
    Loading amount 20 µg
    Specification Mouse prefrontal cortex
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Sample Human Tissue sections (Lymph node)
    Specification Lymph node
    Permeabilization No
    Fixative Acetone

    Abcam user community

    Verified customer

    Submitted Jul 15 2013

    DISCOUNT CODE: 42636
    Expiration date:15-Mar-13

    I am very pleased to hear a customer would like to accept our offer and test ab76543 in IHC-Fr. To redeem this offer, an Abreview needs to be submitted for IHC-Fr on human samples and this cod...

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