Overview

  • Product nameAnti-PUF60 antibody
    See all PUF60 primary antibodies
  • Description
    Rabbit polyclonal to PUF60
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 346 and 533 of Human PUF60 isoform b (NP_055096)

  • Positive control
    • 293T, H1299, and HeLaS3 cell lysates. HepG2, Molt-4, and Raji whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.

Target

  • FunctionDNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
  • Tissue specificityIsoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the RRM half pint family.
    Contains 3 RRM (RNA recognition motif) domains.
  • DomainThe third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions.
  • Cellular localizationNucleus. Colocalizes partially with TROVE2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa poly(U)-binding-splicing factor antibody
    • FBP-interacting repressor antibody
    • FIR antibody
    • FLJ31379 antibody
    • FUSE-binding protein-interacting repressor antibody
    • Poly U binding splicing factor 60KDa antibody
    • Poly(U)-binding-splicing factor PUF60 antibody
    • poly-U binding splicing factor PUF60 antibody
    • PUF60 antibody
    • PUF60_HUMAN antibody
    • pyrimidine tract binding splicing factor antibody
    • Ro ribonucleoprotein-binding protein 1 antibody
    • Ro-binding protein 1 antibody
    • RoBP1 antibody
    • RoBPI antibody
    • siah binding protein 1 antibody
    • Siah-binding protein 1 antibody
    • Siah-BP1 antibody
    • SIAHBP1 antibody
    see all

Anti-PUF60 antibody images

  • All lanes : Anti-PUF60 antibody (ab97335) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa

References for Anti-PUF60 antibody (ab97335)

ab97335 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"