Anti-PUMA alpha antibody - C-terminal (ab182788)

Overview

  • Product name
    Anti-PUMA alpha antibody - C-terminal
  • Description
    Rabbit polyclonal to PUMA alpha - C-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human PUMA alpha aa 180-193 (C terminal).
    Sequence:

    PLPRGHRAPEMEPN


    Database link: Q9BXH1

  • Positive control
    • NIH3T3, A549 and Hela cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab182788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Target

  • Relevance
    PUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. PUMA is indeed an essential mediator of p53-dependent and -independent apoptosis in vivo, mediates the apoptotic response to p53 in colorectal cancer cells and is degraded during Chlamydia trachomatis infection
  • Cellular localization
    Cytoplasmic and Mitochondrial
  • Database links
  • Alternative names
    • bbc3 antibody
    • BCL2 binding component 3 antibody
    • JFY1 antibody
    • p53 upregulated modulator of apoptosis antibody
    • PUMA antibody
    • PUMA/JFY1 antibody
    see all

Images

  • All lanes : Anti-PUMA alpha antibody - C-terminal (ab182788) at 1/540 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti Rabbit IgG at 1/10000 dilution

    Predicted band size : 21 kDa


    Exposure time : 1 minute

References

ab182788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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