Overview

  • Product nameAnti-PUMA antibody
    See all PUMA primary antibodies
  • Description
    Rabbit polyclonal to PUMA
  • SpecificitySpecific to PUMA alpha
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    ARARQEGSSPEPVEG

    , corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646).

  • Positive control
    • K562 lysate, human breast carcinoma sections
  • General notes


    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa.Can be blocked with Human PUMA peptide (ab9646).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

Anti-PUMA antibody images

  • Anti-PUMA antibody (ab9645) at 2 µg/ml + human K562 cell lysate

    Performed under reducing conditions.
  • Immunohistochemistry of PUMA in human breast carcinoma with ab9645 at 10 ug/ml.

References for Anti-PUMA antibody (ab9645)

This product has been referenced in:
  • Palomo GM  et al. Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells. Hum Mol Genet 20:2807-22 (2011). Read more (PubMed: 21531789) »
  • Höglund A  et al. Myc sensitizes p53-deficient cancer cells to the DNA-damaging effects of the DNA methyltransferase inhibitor decitabine. Blood 113:4281-8 (2009). WB, IF ; Human, Mouse . Read more (PubMed: 19179467) »

See all 8 Publications for this product

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