Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab81825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
  • Tissue specificity
    Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Developmental stage
    Expression in neutrophils occurs in the late terminal differentiation phase.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 109A antibody
    • G protein coupled receptor HM74a antibody
    • G protein coupled receptor Hm74b antibody
    • G-protein coupled receptor 109A antibody
    • G-protein coupled receptor HM74A antibody
    • G109A_HUMAN antibody
    • GPR 109A antibody
    • Gpr109a antibody
    • GPR109A protein antibody
    • HCA2 antibody
    • HCAR2 antibody
    • HM 74 antibody
    • HM 74a antibody
    • HM 74b antibody
    • Hm74 antibody
    • HM74a antibody
    • HM74b antibody
    • hydroxycarboxylic acid receptor 2 antibody
    • Interferon gamma inducible gene Puma g antibody
    • Niacin receptor 1 antibody
    • NIACR1 antibody
    • Nicotinic acid receptor 1 antibody
    • Nicotinic acid receptor antibody
    • Protein upregulated in macrophages by IFNG, mouse, homolog of antibody
    • Puma g antibody
    • Pumag antibody
    • rHM74b antibody
    see all

Images

  • Anti-Puma gamma antibody (ab81825) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 12 minutes
  • ICC/IF image of ab81825 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 rabbit anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab81825 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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