Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.
Our Abpromise guarantee covers the use of ab3717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21865157|
|WB||1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).|
|IP||Use at 1-5 µg/mg of lysate.|
Sample: Hela cell total RIPA extract (10
Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)
CIP: Calf Intestinal Phosphatase
Detection: ECL exposure for 1 minute.