Overview

  • Product nameAnti-Pumilio 1 antibody
    See all Pumilio 1 primary antibodies
  • Description
    Rabbit polyclonal to Pumilio 1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Human, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Pumilio 1.

  • Positive control
    • This antibody gave a positive signal in Mouse lung tissue lysate in western blot and MCF7 whole cells in Immunofluorescence.

Properties

Applications

Our Abpromise guarantee covers the use of ab97993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 127 kDa).

Target

Anti-Pumilio 1 antibody images

  • ICC/IF image of ab97993 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97993 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293,and HepG2 cells at 5ug/ml.

  • Anti-Pumilio 1 antibody (ab97993) at 1 µg/ml + Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 127 kDa
    Observed band size : 147 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa,34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Pumilio 1 antibody (ab97993)

ab97993 has not yet been referenced specifically in any publications.

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