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Rabbit monoclonal [EPR3795] to Pumilio 1
This antibody is predicted to not cross react with Pumilio 2.
Synthetic peptide. within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
Database link: Q14671
WB: HeLa and 293T cell lysates
IHC-P: Human fetal kidney tissue
ICC/IF: HeLa cells
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/1000 - 1/5000. Predicted molecular weight: 126 kDa.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Information by UniProt
PUM 1 antibody
Anti-Pumilio 1 antibody [EPR3795] images
Western blot - Pumilio 1 antibody [EPR3795] (ab92545)
All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysates Lysates/proteins at 10 µg per lane. Secondary Standard HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 126 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pumilio 1 antibody [EPR3795] (ab92545)
Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Pumilio 1 antibody [EPR3795] (ab92545)
Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.
Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] (ab92545)
Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x10
cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Pumilio 1 antibody [EPR3795] (ab92545)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"