Anti-Pumilio 1 antibody [EPR3795] (ab92545)

Overview

  • Product nameAnti-Pumilio 1 antibody [EPR3795]
    See all Pumilio 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3795] to Pumilio 1
  • SpecificityThis antibody is predicted to not cross react with Pumilio 2.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
    Database link: Q14671

  • Positive control
    • WB: HeLa and 293T cell lysates IHC-P: Human fetal kidney tissue ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 126 kDa.
IP 1/50.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

Anti-Pumilio 1 antibody [EPR3795] images

  • All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 126 kDa
  • Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.
  • Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.
  • Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Pumilio 1 antibody [EPR3795] (ab92545)

ab92545 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C antibody (sc-15386) bound protein A dynabeads 0.1 mg/ml (antibody/beads).
Negative control Protein A dynabeads without antibody bound.
Immuno-precipitation step Other - Protein A dynabeads
Username

Abcam user community

Verified customer

Submitted Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"