Anti-Pumilio 1 antibody - N-terminal (ab75943)

Overview

  • Product nameAnti-Pumilio 1 antibody - N-terminal
    See all Pumilio 1 primary antibodies
  • Description
    Rabbit polyclonal to Pumilio 1 - N-terminal
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated Synthetic peptide: AMGEQLLPGK KFWETDESSK DGPKGIFGD QWRDSAWGTS DHSVSQPIMV, corresponding to N terminal amino acids 140-190 of Human Pumilio 1

  • Positive control
    • WB: lysate from 293 cells, transfected with the PUM1 gene; IHC: testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab75943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 126 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

Anti-Pumilio 1 antibody - N-terminal images

  • All lanes : Anti-Pumilio 1 antibody - N-terminal (ab75943) at 1/50 dilution

    Lane 1 : 293 cell lysates, non-transfected
    Lane 2 : 293 cell lysates, transiently transfected with the PUM1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 126 kDa
  • Staining of formalin-fixed, paraffin-embedded human testis tissue using ab75943 at 1/10-1/50 dilution, in conjunction with a peroxidase-conjugated secondary antibody, followed by DAB staining.

References for Anti-Pumilio 1 antibody - N-terminal (ab75943)

ab75943 has not yet been referenced specifically in any publications.

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