Anti-Pumilio 2 antibody (ab10361)
Key features and details
- Rabbit polyclonal to Pumilio 2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Pumilio 2 antibody
See all Pumilio 2 primary antibodies -
Description
Rabbit polyclonal to Pumilio 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human Pumilio 2 aa 100-200.
Database link: 23369 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa).
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IP |
Use at 5-10 µg/mg of lysate.
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Notes |
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WB
1/10000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa). |
IP
Use at 5-10 µg/mg of lysate. |
Target
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Function
Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. -
Tissue specificity
Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. -
Sequence similarities
Contains 1 PUM-HD domain.
Contains 8 pumilio repeats. -
Domain
The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs. -
Cellular localization
Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. - Information by UniProt
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Database links
- Entrez Gene: 282441 Cow
- Entrez Gene: 23369 Human
- Entrez Gene: 80913 Mouse
- Omim: 607205 Human
- SwissProt: Q8TB72 Human
- SwissProt: Q80U58 Mouse
- Unigene: 467824 Human
- Unigene: 341243 Mouse
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Alternative names
- FLJ36528 antibody
- KIAA0235 antibody
- MGC138251 antibody
see all
Images
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All lanes : Anti-Pumilio 2 antibody (ab10361) at 1/5000 dilution
Lane 1 : HeLa Whole cell lysate at 5 µg
Lane 2 : HeLa Whole cell lysate at 15 µg
Lane 3 : HeLa Whole cell lysate at 50 µg
Lane 4 : HEK293T Whole cell lysate at 50 µg
Lane 5 : NIH 3T3 Whole cell lysate at 50 µg
Predicted band size: 127 kDaDetection: Chemiluminescence with exposure time of 10 seconds
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Pumilio 2 was immunoprecipitated from HeLa whole cell lysate with 3 µg/mg lysate ab10361. Western blot was performed from the immunoprecipitate using ab10361 at 1 µg/ml dilution.
Lane 1: ab10361 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab10361 has been referenced in 9 publications.
- Mulroe F et al. Targeting firing rate neuronal homeostasis can prevent seizures. Dis Model Mech 15:N/A (2022). PubMed: 36073607
- Wang Y et al. PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression. Cell Struct Funct 44:29-39 (2019). PubMed: 30787206
- Zhang L et al. RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a. Biomed Pharmacother 114:108772 (2019). PubMed: 30909144
- D'Amico D et al. The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging. Mol Cell 73:775-787.e10 (2019). PubMed: 30642763
- Khong A et al. The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules. Mol Cell 68:808-820.e5 (2017). PubMed: 29129640
- Marrero E et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). WB, IHC-Fr ; Mouse . PubMed: 21865157
- Vessey JP et al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 107:3222-7 (2010). WB . PubMed: 20133610
- Barrios F et al. Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells. J Cell Sci 123:871-80 (2010). PubMed: 20159962
- Lolicato F et al. Potential role of Nanos3 in maintaining the undifferentiated spermatogonia population. Dev Biol 313:725-38 (2008). PubMed: 18089289