Overview

  • Product nameAnti-Pumilio 2 antibody
    See all Pumilio 2 primary antibodies
  • Description
    Goat polyclonal to Pumilio 2
  • Specificityab32894 recognises PUM2.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-DQNRDLKQGDDDDSK

    , corresponding to Internal sequence amino acids 141-155 of Human Pumilio 2

  • Positive control
    • Human Epstein-Barr virus immortilised lymphoblastoid lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
WB Use a concentration of 0.3 - 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 114 kDa).

Target

  • FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similaritiesContains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Anti-Pumilio 2 antibody images

  • All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/ml

    Lane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptide
    Lane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptide

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Lane 1 : Anti-Pumilio 2 antibody (ab32894) at 0.5 µg/ml
    Lane 2 : Anti-Pumilio 2 antibody (ab32894) at 1 mg/ml
    Lane 3 : anti- DYKDDDDK Tag at 1/3000 dilution

    Lane 1 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µg
    Lane 2 : Mock Tansfected HEK293
    Lane 3 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µg


    Predicted band size : 114 kDa

References for Anti-Pumilio 2 antibody (ab32894)

ab32894 has not yet been referenced specifically in any publications.

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