• Product nameAnti-Pumilio 2 antibody
    See all Pumilio 2 primary antibodies
  • Description
    Rabbit polyclonal to Pumilio 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: RFRKGNFGTR DAETDGPEKG DQKGKASPFE EDQNRDLKQG DDDDSKINGR , corresponding to amino acids 110-159 of Human Pumilio 2

  • Positive control
    • Pumilio 2 transfected 293T cell lysate.



Our Abpromise guarantee covers the use of ab66669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similaritiesContains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Anti-Pumilio 2 antibody images

  • Anti-Pumilio 2 antibody (ab66669) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 114 kDa
    Observed band size : 114 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Pumilio 2 antibody (ab66669)

ab66669 has not yet been referenced specifically in any publications.

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