Anti-Pumilio 2 antibody [EPR3813] (ab92390)

Overview

  • Product nameAnti-Pumilio 2 antibody [EPR3813]
    See all Pumilio 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3813] to Pumilio 2
  • SpecificityThis antibody is predicted to not cross react with Pumilio 1.
  • Tested applicationsSuitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Pumilio 2

  • Positive control
    • HeLa and 293T cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC
  • Application notesICC: 1/100 - 1/250.

    IP: 1/10.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 114 kDa.

    Is unsuitable for Flow Cyt or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
    • Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
    • Sequence similaritiesContains 1 PUM-HD domain.
      Contains 8 pumilio repeats.
    • DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
    • Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36528 antibody
      • KIAA0235 antibody
      • MGC138251 antibody
      • MGC138253 antibody
      • PUM 2 antibody
      • PUM2 antibody
      • PUM2_HUMAN antibody
      • PUMH 2 antibody
      • PUMH2 antibody
      • Pumilio (Drosphila) homolog 2 antibody
      • Pumilio homolog 2 (Drosophila) antibody
      • Pumilio homolog 2 antibody
      • Pumilio-2 antibody
      • Pumilio2 antibody
      • PUML2 antibody
      see all

    Anti-Pumilio 2 antibody [EPR3813] images

    • All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 114 kDa
      Observed band size : 114 kDa

    References for Anti-Pumilio 2 antibody [EPR3813] (ab92390)

    ab92390 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92390.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"