Recombinant Anti-Pumilio 2 antibody [EPR3813] (ab92390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3813] to Pumilio 2
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Pumilio 2 antibody [EPR3813]
See all Pumilio 2 primary antibodies -
Description
Rabbit monoclonal [EPR3813] to Pumilio 2 -
Host species
Rabbit -
Specificity
This antibody is predicted to not cross react with Pumilio 1. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Pumilio 2 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: HeLa and 293T cell lysates ICC/IF: MCF-7 cells. Flow Cyt (intra): HeLa cells IP: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3813 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/30.
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WB |
1/5000 - 1/10000. Predicted molecular weight: 114 kDa.
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IP |
1/10 - 1/20.
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ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250. |
Notes |
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Flow Cyt (Intra)
1/30. |
WB
1/5000 - 1/10000. Predicted molecular weight: 114 kDa. |
IP
1/10 - 1/20. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
Target
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Function
Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. -
Tissue specificity
Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. -
Sequence similarities
Contains 1 PUM-HD domain.
Contains 8 pumilio repeats. -
Domain
The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs. -
Cellular localization
Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. - Information by UniProt
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Database links
- Entrez Gene: 23369 Human
- Entrez Gene: 80913 Mouse
- Entrez Gene: 298874 Rat
- Omim: 607205 Human
- SwissProt: Q8TB72 Human
- SwissProt: Q80U58 Mouse
- Unigene: 467824 Human
- Unigene: 341243 Mouse
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Alternative names
- FLJ36528 antibody
- KIAA0235 antibody
- MGC138251 antibody
see all
Images
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All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa -
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/50 dilution (4.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/30 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab92390 (purified) at 1/20 dilution (1ug) immunoprecipitating Pumilio 2 in HeLa whole cell lysates.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): ab92390 & HeLa whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92390 in HeLa whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab92390 has been referenced in 12 publications.
- Liu Q et al. PUM2 aggravates the neuroinflammation and brain damage induced by ischemia-reperfusion through the SLC7A11-dependent inhibition of ferroptosis via suppressing the SIRT1. Mol Cell Biochem 478:609-620 (2023). PubMed: 35997855
- Liu Z & Lv C RNA binding protein PUM2 promotes hepatocellular carcinoma proliferation and apoptosis via binding to the 3'UTR of BTG3. Oncol Lett 24:346 (2022). PubMed: 36072004
- Gong Y et al. PUMILIO proteins promote colorectal cancer growth via suppressing p21. Nat Commun 13:1627 (2022). PubMed: 35338151
- Xu L et al. Downregulated expression levels of USP46 promote the resistance of ovarian cancer to cisplatin and are regulated by PUM2. Mol Med Rep 23:N/A (2021). PubMed: 33576437
- Shen L et al. LncRNA TTN-AS1 promotes endometrial cancer by sponging miR-376a-3p. Oncol Rep 44:1343-1354 (2020). PubMed: 32945477