Overview

  • Product nameAnti-Punt antibody
  • Description
    Rabbit polyclonal to Punt
  • Tested applicationsSuitable for: ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide:

    QLLIKNHTNIDDAKESTNC

    , corresponding to C terminal amino acids 497-515 of Drosophila melanogaster Punt. Peptides were amidated for conjugation.

  • Positive control
    • D. melanogaster extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab14680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
IP
WB
  • Application notesICC: Use at an assay dependent dilution.
    IP: >1/200.
    WB: >1/500. Detects a band of approximately 63 kDa

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog and wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promotes its cell division in the female germline. The Signaling by TGF beta related factors requires ligand induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor is encoded by the thick veins (tkv) gene and the type II receptor is encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required to block the development of various organelles including salivary glands. These are Mothers against dpp (Mad), Medea (Med) and Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development. Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp dependent patterning process. No Dpp dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling.
    • Database links
      • Alternative names
        • Activin receptor type II antibody
        • Atr antibody
        • Pun antibody
        • Put antibody
        • STKC antibody
        • TGF B antibody
        • TGF beta antibody
        see all

      Anti-Punt antibody images

      • All lanes : Anti-Punt antibody (ab14680) at 1/1000 dilution

        Lane 1 : 0-4 hour old EGFP shRNAi embryos (Control)
        Lane 2 : 0-4 hour old put shRNAi embryos

        Lysates/proteins at 30 µg per lane.

        Secondary
        Amersham ECL anti-Rabbit IgG, HRP conjugated at 1/1000 dilution
        Developed using the ECL technique

        Performed under reducing conditions.

        Predicted band size : 62 kDa
        Observed band size : 65 kDa (why is the actual band size different from the predicted?)
        Additional bands at : 180 kDa (possible non-specific binding),38 kDa (possible non-specific binding),40 kDa (possible non-specific binding),55 kDa (possible non-specific binding),90 kDa (possible non-specific binding).

        Exposure time : 4 minutes

        Image courtesy of Dr. Richelle Sopko, Harvard Medical School, U.S.

        See Abreview

      References for Anti-Punt antibody (ab14680)

      ab14680 has not yet been referenced specifically in any publications.

      Product Wall

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Western blot
      Loading amount 30 µg
      Gel Running Conditions Reduced Denaturing (4-20% gel)
      Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
      Specification 0-4hr embryo
      Treatment control shRNA and punt shRNA embryos
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Username

      Dr. Richelle Sopko

      Verified customer

      Submitted Aug 01 2013

      Abcam has not validated the combination of species/application used in this Abreview.
      Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample Fruit fly (Drosophila melanogaster) Tissue sections (ovary and eye disc)
      Specification ovary and eye disc
      Fixative Paraformaldehyde
      Antigen retrieval step None
      Permeabilization No
      Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Username

      Dr. Zhiyan Liu

      Verified customer

      Submitted Jul 15 2010

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"