Overview

  • Product nameAnti-PURA antibody
    See all PURA primary antibodies
  • Description
    Rabbit polyclonal to PURA
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human PURA (NP_005850.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    Anti-PURA antibody images

    • Detection of PURA by Western Blot of Immunprecipitate. Lane 1; an antibody to PURA at 1µg/ml staining PURA in HeLa whole cell lysate immunoprecipitated using ab125200 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 10 seconds.

    References for Anti-PURA antibody (ab125200)

    ab125200 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Mouse Tissue lysate - nuclear (Brain)
    Total protein in input 4 µg
    Specification Brain
    Immuno-precipitation step Other - Protein g DYNABEADS
    Username

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    Verified customer

    Submitted Jan 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"