Anti-PURA antibody (ab77734)

Overview

  • Product nameAnti-PURA antibody
    See all PURA primary antibodies
  • Description
    Mouse monoclonal to PURA
  • Tested applicationsSuitable for: WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 183-292 of Human PURA (NP_005850) with a tag of 26 KDa.

  • Positive control
    • Human skeletal muscle lysate. Recombinant tagged human PURA fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged PURA is approximately 0.3ng/ml when used as a capture antibody.
ICC Use at an assay dependent concentration.

Target

  • FunctionThis is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
  • Sequence similaritiesBelongs to the PUR DNA-binding protein family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • PUR ALPHA antibody
    • PUR1 antibody
    • purA antibody
    • PURA_HUMAN antibody
    • PURALPHA antibody
    • Purine rich element binding protein A antibody
    • Purine rich single stranded DNA binding protein alpha antibody
    • Purine-rich single-stranded DNA-binding protein alpha antibody
    • Transcriptional activator protein PUR alpha antibody
    • Transcriptional activator protein Pur-alpha antibody
    see all

Anti-PURA antibody images

  • Anti-PURA antibody (ab77734) at 1 µg/ml + Recombinant tagged human PURA fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-PURA antibody (ab77734) at 1 µg/ml + human skeletal muscle lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Detection limit for recombinant tagged PURA is approximately 0.3ng/ml as a capture antibody.
  • ab77734 staining PURA in mouse primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol prior to blocking in 1% BSA for 1 hour at 22°C. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at 22°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/500.

    See Abreview

References for Anti-PURA antibody (ab77734)

ab77734 has not yet been referenced specifically in any publications.

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Sep 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"