Overview

  • Product nameAnti-PURA antibody
    See all PURA primary antibodies
  • Description
    Rabbit polyclonal to PURA
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PURA.

    (Peptide available as ab88160.)

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab79936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionThis is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
  • Sequence similaritiesBelongs to the PUR DNA-binding protein family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • PUR ALPHA antibody
    • PUR1 antibody
    • purA antibody
    • PURA_HUMAN antibody
    • PURALPHA antibody
    • Purine rich element binding protein A antibody
    • Purine rich single stranded DNA binding protein alpha antibody
    • Purine-rich single-stranded DNA-binding protein alpha antibody
    • Transcriptional activator protein PUR alpha antibody
    • Transcriptional activator protein Pur-alpha antibody
    see all

Anti-PURA antibody images

  • Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.

  • ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.

References for Anti-PURA antibody (ab79936)

This product has been referenced in:
  • Moldovan JB & Moran JV The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet 11:e1005121 (2015). Read more (PubMed: 25951186) »
  • Xu Z  et al. Expanded GGGGCC repeat RNA associated with amyotrophic lateral sclerosis and frontotemporal dementia causes neurodegeneration. Proc Natl Acad Sci U S A 110:7778-83 (2013). WB . Read more (PubMed: 23553836) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Cell lysate - whole cell (whole brain)
Specification whole brain
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jul 10 2013

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed agains...

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Thank you for contacting Abcam. I am sorry that you have been experiencing difficulties with our two antibodies, ab14744 and ab77734.  We have received positive feedback for both of these antibodies in ICC and so I am not sure why they have not wor...

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Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
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Submitted Jun 02 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cultured Cells (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0,25% Triton in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 30 2010

Application Western blot
Sample Rat Tissue lysate - whole (brain lysate differentially centrifuged)
Loading amount 5 µg
Specification brain lysate differentially centrifuged
Gel Running Conditions Reduced Denaturing (12 % SDS Gel)
Blocking step Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 30 2010

Application Immunocytochemistry
Sample Mouse Cultured Cells (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
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Submitted Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"