Overview

  • Product name
  • Description
    Rabbit polyclonal to PUS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 124-404 of Human PUS1 (Uniprot ID: Q9Y606).

  • Positive control
    • 293T, H1299, HeLa, HepG2, MOLT4 and Raji cell lines; A431 and Mouse brain whole cell lysate; A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function
    Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
  • Tissue specificity
    Widely expressed. High levels of expression found in brain and skeletal muscle.
  • Involvement in disease
    Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
  • Sequence similarities
    Belongs to the tRNA pseudouridine synthase TruA family.
  • Cellular localization
    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A730013B20Rik antibody
    • DOBI antibody
    • MGC112655 antibody
    • MGC11268 antibody
    • MLASA antibody
    • MLASA1 antibody
    • mPus1p antibody
    • Pseudouridine synthase 1 antibody
    • Pseudouridylate synthase 1 antibody
    • PUS1 antibody
    • tRNA pseudouridine synthase A, mitochondrial antibody
    • tRNA pseudouridine(38-40) synthase antibody
    • tRNA pseudouridylate synthase I antibody
    • tRNA uridine isomerase I antibody
    • tRNA-uridine isomerase I antibody
    • TRUA_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse muscle tissue labeling PUS1 with ab155544 at 1/500 dilution.

  • Anti-PUS1 antibody (ab155544) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Anti-PUS1 antibody (ab155544) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling PUS1 with ab155544 at 1/500 dilution.

References

ab155544 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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