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Rabbit polyclonal to PUS1
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between amino acids 377 and 427 of human PUS1 (NP_079491.2).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab85938 was affinity purified using an epitope specific to PUS1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
Widely expressed. High levels of expression found in brain and skeletal muscle.
Involvement in disease
Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
Belongs to the tRNA pseudouridine synthase TruA family.
Mitochondrion and Nucleus.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS1 antibody (ab85938)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PUS1 with ab85938 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - PUS1 antibody (ab85938)
All lanes : Anti-PUS1 antibody (ab85938) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique Predicted band size : 47 kDa Observed band size : 47 kDa Exposure time : 10 secondsNote: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
Immunoprecipitation - PUS1 antibody (ab85938)
Detection of human PUS1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85938 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
has not yet been referenced specifically in any publications.
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