• Product nameAnti-PVRL1 antibody
  • Description
    Rabbit polyclonal to PVRL1
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PVRL1.

    (Peptide available as ab73475.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue; Testis (Human) Tissue; HeLa Whole Cell; SK N SH Whole Cell; Hela Whole Cell - Staurosporine Treated (24hr, 500nM)



Our Abpromise guarantee covers the use of ab66985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa). Abcam recommends using milk as the blocking agent.
IHC-P Use at an assay dependent concentration. PubMed: 24130898
IHC-Fr Use at an assay dependent concentration. PubMed: 21769484


  • FunctionPromotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
  • Involvement in diseaseDefects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.
    Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similaritiesBelongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD111 antibody
    • CD111 antigen antibody
    • CLPED1 antibody
    • ectodermal dysplasia 4 (Margarita Island type) antibody
    • ED4 antibody
    • Herpes virus entry mediator C antibody
    • Herpesvirus entry mediator C antibody
    • Herpesvirus Ig like receptor antibody
    • Herpesvirus Ig-like receptor antibody
    • HIgR antibody
    • HveC antibody
    • MGC142031 antibody
    • Nectin 1 antibody
    • Nectin-1 antibody
    • OFC7 antibody
    • OROFACIAL CLEFT 7 antibody
    • OTTHUMP00000232093 antibody
    • OTTHUMP00000232094 antibody
    • OTTHUMP00000232095 antibody
    • Poliovirus receptor related protein 1 antibody
    • poliovirus receptor-like 1 antibody
    • Poliovirus receptor-related protein 1 antibody
    • PRR antibody
    • PRR1 antibody
    • PVRL 1 antibody
    • PVRL1 antibody
    • PVRL1_HUMAN antibody
    • PVRR antibody
    • PVRR1 antibody
    • SK-12 antibody
    see all

Anti-PVRL1 antibody images

  • All lanes : Anti-PVRL1 antibody (ab66985) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Dorsal Root Ganglion
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 210 kDa,35 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    PVRL1 protein is known to be glycosylated and it migrates at a higher molecular weight than the predicted band size of 57kDa (Swiss-prot data), as demonstrated by PMID 15731277. Abcam recommends using 3% milk as the blocking agent with ab66985
  • IHC-FoFr image of PVRL1 (ab66985) staining on Rat hippocampus and Striatum sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References for Anti-PVRL1 antibody (ab66985)

This product has been referenced in:
  • Gillory LA  et al. Preclinical evaluation of engineered oncolytic herpes simplex virus for the treatment of neuroblastoma. PLoS One 8:e77753 (2013). IHC-P ; Human . Read more (PubMed: 24130898) »
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010