Recombinant
RabMAb

Anti-PWP1 antibody [EPR16130] (ab190794)

Overview

  • Product name
    Anti-PWP1 antibody [EPR16130]
    See all PWP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR16130] to PWP1
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PWP1 aa 50-150. The exact sequence is proprietary.
    Database link: Q13610

  • Positive control
    • Jurkat, HeLa, 293, HepG2 cell lysates, Human pancreas tissue, Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16130
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    May play an important role in cell growth and/or transcription.
  • Tissue specificity
    High levels seen in the placenta, skeletal muscle, kidney and pancreas while lower levels were seen in the heart, brain and lung.
  • Sequence similarities
    Belongs to the WD repeat PWP1 family.
    Contains 5 WD repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Keratinocyte protein IEF SSP 9502 antibody
    • Periodic tryptophan protein 1 homolog antibody
    • PWP1 antibody
    • PWP1 homolog antibody
    • PWP1_HUMAN antibody
    see all

Images

  • Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 56 kDa
    Additional bands at : 79 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 56 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

  • Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

References

ab190794 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190794.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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