Overview

  • Product name
  • Description
    Mouse polyclonal to PWP2H
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PWP2H (NP_005040.2, 1 a.a. ~ 919 a.a) full-length human protein.

  • Positive control
    • HeLa cells, transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml. Antibody must be purified before use.
    WB: 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the WD repeat PWP2 family.
      Contains 14 WD repeats.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EHOC 17 antibody
      • Periodic tryptophan protein 2 homolog antibody
      • pwp2 antibody
      • PWP2 periodic tryptophan protein homolog antibody
      • PWP2 periodic tryptophan protein homolog (yeast) antibody
      • PWP2_HUMAN antibody
      see all

    Anti-PWP2H antibody images

    • All lanes : Anti-PWP2H antibody (ab67848) at 1/500 dilution

      Lane 1 : PWP2H transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 102 kDa
      Observed band size : 102 kDa
    • Immunofluorescence analysis of HeLa cells labelled with purified ab67848 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed using 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.

    References for Anti-PWP2H antibody (ab67848)

    This product has been referenced in:
    • Gallenberger M  et al. Lack of WDR36 leads to preimplantation embryonic lethality in mice and delays the formation of small subunit ribosomal RNA in human cells in vitro. Hum Mol Genet 20:422-35 (2011). ICC/IF ; Human . Read more (PubMed: 21051332) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab67848.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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